Septins-Regulate-Bacterial-Entry-into-Host-Cells-pone.0004196.s002

Description:

Description: English: Representative FRET movie of a control siRNA-treated cell. HeLa cells treated with control (CTRL) siRNA were transfected with YFP-AktPH and CFP-AktPH. Transfected siRNA-treated cells were placed on the microscope stage at 37°C and image series were collected every 15 seconds for 15 minutes. Purified InlB was added to a final concentration of 5 nM after frame 15 (i.e. time point just prior to 4 minutes) after the start of imaging. A movie for the entire timecourse of InlB stimulation for the control cell depicted in Figure 5A is here presented (i.e. frame 0 to 60), showing the collected images (phase-contrast, Akt recruitment, and FRET efficiency). Pseudocolor scale represents the range of FRET efficiency values from original signal ranging from blue (low) to red (high). Date: 2009. Source: Movie S2 from Mostowy S, Nam Tham T, Danckaert A, Guadagnini S, Boisson-Dupuis S, Pizarro-Cerdá J, Cossart P (2009). "Septins Regulate Bacterial Entry into Host Cells". PLOS ONE. DOI:10.1371/journal.pone.0004196. PMID 19145258. PMC: 2626286. Author: Mostowy S, Nam Tham T, Danckaert A, Guadagnini S, Boisson-Dupuis S, Pizarro-Cerdá J, Cossart P. Permission (Reusing this file): : This file is licensed under the Creative Commons Attribution 2.5 Generic license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue. This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.. Provenance: This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.: .