Description: English: A video taken from a confocal stack. The video from a confocal stack (Figure 13Aii) was imaged from the ventral surface through to the mid-brain of a P. clarkii brain at hatching (approximately 26 μm into the brain tissue). There are three labels: (1) dextran dye (green) micro-injected into the cerebral artery via the dorsal sinus combined with (2) the S-phase marker BrdU (red) identifying the cap and the immunolocalization of (3) tyrosinated tubulin (white) delineating the protoniche and its pore. The video shows that the cerebral artery bifurcates and then pushes ventrally toward the two hemispheres, each branch dividing laterally and then many times over into the neuropile regions as continuous tubes. The protoniche has blood vessels confluent with its structure and connects to blood vessels slightly deeper into the tissue. The central pore, considered as an early stage of the cavity, is confluent with the blood vasculature. Date: 2012. Source: Sintoni S, Benton J, Beltz B, Hansson B, Harzsch S (2012). "
Neurogenesis in the central olfactory pathway of adult decapod crustaceans: development of the neurogenic niche in the brains of procambarid crayfish". Neural Development.
DOI:
10.1186/1749-8104-7-1.
PMID 22225949.
PMC:
3266201. Author: Sintoni S, Benton J, Beltz B, Hansson B, Harzsch S. Permission (
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