Description: English: Representative FRET movie of a SEPT2-depleted cell. HeLa cells treated with siRNA targeted against SEPT2 were transfected with YFP-AktPH and CFP-AktPH. Transfected siRNA-treated cells were placed on the microscope stage at 37°C and image series were collected every 15 seconds for 15 minutes. Purified InlB was added to a final concentration of 5 nM after frame 15 (i.e. time point just prior to 4 minutes) after the start of imaging. A movie for the entire timecourse of InlB stimulation for the SEPT2-depleted cell depicted in Figure 5A is here presented (i.e. frame 0 to 60), showing the collected images (phase-contrast, Akt recruitment, and FRET efficiency). Pseudocolor scale represents the range of FRET efficiency values from original signal ranging from blue (low) to red (high). Date: 2009. Source: Movie S3 from Mostowy S, Nam Tham T, Danckaert A, Guadagnini S, Boisson-Dupuis S, Pizarro-Cerdá J, Cossart P (2009). "
Septins Regulate Bacterial Entry into Host Cells". PLOS ONE.
DOI:
10.1371/journal.pone.0004196.
PMID 19145258.
PMC:
2626286. Author: Mostowy S, Nam Tham T, Danckaert A, Guadagnini S, Boisson-Dupuis S, Pizarro-Cerdá J, Cossart P. Permission (
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